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Osteoclast differentiation factor Denosumab (DMab) is a human monoclonal IgG2 antibody that inhibits bone resorption by binding and inhibiting the receptor activator of the NF-kB ligand (RANKL), an essential cytokine for osteoclastogenesis and bone resorption

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As a positive control for apoptosis, tamoxifen (50 M) was included ( 2 , 1.2 mM EGTA, 1.5% Nonidet P40, 0.3% CHAPS, 30% sucrose, 150 M caspase 3 substrate DEVD-AMC, 30 mM DTT, and 3.0 mM PMSF was directly added to HepG2 cells