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Eli Lillys explanation about who it will consider for expanded access broadly follows the widely held understanding of the term, including the stipulation that eligible patients must have a serious or life-threatening illness with no comparable or satisfactory alternative therapies. Thats partly why the decision to grant expanded access for a medication primarily known for weight loss raised so many questions

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Flow cytometry Briefly, cells were collected by centrifugation, resuspended, and stained with 5 L Annexin V-APC and/or 5 L propidium iodide (PI) (Sigma)

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Likewise, BPC 157 counteracted disturbances caused by reserpine application and thereby vesicle depletion [30]