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Superoxide anion (O 2 - ), hydroxyl (OH ), alkoxy radicals (RO ), peroxyl radicals (ROO ), nitric oxide radical (NO ), nitrogen dioxide (NO 2 ), and carbonate radical (CO 3 - ) are the few examples of radical ROS whereas non-radical ROS include singlet oxygen ( 1 O 2 ), hydrogen peroxide (H 2 O 2 ), hypochlorous acid (HOCl), and peroxynitrite (ONOO - ) species which can readily trigger free radical reactions in organisms [23, 24]

Discrimination between viable and dead cells was achieved by staining with 5 M calcein AM and 0.3 M DRAQ 7, as described in the Single-Cell Capture and cDNA Synthesis Protocol for BD Rhapsody (BD Bioscience)

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Compound 1: GHK-Cu Research Findings Cellular Fibroblast collagen, elastin, glycosaminoglycan, decorin, and dermatan sulfate synthesis