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In addition to energy production, mitochondria perform other cellular functions: Transport and storage of calcium ions used for cellular processes like muscle contraction and signal transduction Release of certain proteins to initiate programmed cell death Regulation of cell differentiation and growth Synthesis of heme, a molecule needed for hemoglobin production hemoglobin carries the oxygen transported by red blood cells Glutathione supports mitochondrial health by neutralizing harmful reactive oxygen species (ROS) that are byproducts of energy metabolism

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Transcriptomic analyses resulting from the bulk RNA-sequencing of the primary cultures also allowed us to identify differentially expressed genes in the 3D vs 2D comparison, with the aim of establishing the molecular mechanisms that orchestrate the phenomenon of ferroptosis resistance in LUAD-CSCs