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Additionally, during the acridine orange (AO) reaction for a lysosome membrane permeability assay, the cells with indicated treatments were stained with 2 g/mL AO (Invitrogen) for 15 min at 37 C

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At the same time, key metabolites in the longissimus dorsi, such as N,N,N-trimethyllysine, L-carnitine, and betaine, along with the key gene MPO , promoted intramuscular fat deposition by inhibiting fat oxidation and promoting fat synthesis (Figs

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Green coffee extract is possibly effective against weight gain and fat accumulation by inhibition of fat absorption and activation of fat metabolism in the liver 2