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Slides were treated with HIER buffer (10 mM sodium citrate, 0.05% Tween-20, pH 6.0) for antigen retrieval in a pressure cooker for 10 min then sections were blocked in CAS-block histochemical reagent (Thermo Fisher Scientific, 008120)

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Blue Raspberry Serving Size: 15 ml Servings Per Container: 32 Amount Per Serving Amt %DV Vitamin B1 (as Thiamine HCI) 1.5mg 130% Vitamin B2 (as Riboflavin) 1.7mg 130% Vitamin B12 (as Methylcobalamin) 6mcg 250% Vitamin B5 (as D-Pantothenic Acid) 5mg 100% * % Daily Value is based on a 2,000 calorie diet

Considering the importance of methionine (MET) as a methyl donor in DNA methylation processes, studies have investigated the effects of RPM supplementation in males on epigenetic regulation, as well as on gene and protein expression profiles related to sperm quality and seminal plasma composition