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Having incubated the immunoprecipitated samples overnight at 4 C with rotation, the anti-M2 FLAG antibody-conjugated agarose beads were washed three times (each for 5 min) with ice-cold washing buffer (50 mM Tris, pH 7.4, 137 mM NaCl, 10% glycerol, 1% NP-40)

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Lactylation and tumors of the nervous system Glioblastoma Gliomas comprise a major fraction of primary brain tumors, with glioblastoma representing the most common malignant subtype in adults and remaining highly lethal despite multimodal therapy [257, 258]

doi: 10.14348/molcells.2015.0258 11 WangQZhangHXuHGuoDShiHLiYet al