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These include weight and age: The way your breasts look when youre a teenager is bound to be different than the way theyll look later in life

doi:10.1007/978-3-319-65536-9_7

The cells in the appropriately labelled wells were used subsequently for messenger ribonucleic acid (mRNA) extraction

Plates were washed twice with ice-cold PBS, scraped, pelleted 250 g at 4 C for 3 min and treated on ice for 5 min with 4 ml of ice-cold HLB + N buffer (10 mM Tris-HCl (pH 7.5), 10 mM NaCl, 2.5 mM MgCl 2 , 0.5% NP-40, 1x phosphatase inhibitor cocktail (Sigma, P0044) and 1x protease inhibitor cocktail (Sigma, P8340))

PMID 19303062