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Retinal cells in culture were loaded for 40 min with 5M Fura-2/AM (Molecular Probes), 0.1% fatty acid-free bovine serum albumin (BSA), and 0.02% pluronic acid F-127 (Molecular Probes) in Krebs solution (132mM NaCl, 4mM KCl, 1.4mM MgCl 2 , 2.5mM CaCl 2 , 6mM glucose, 10mM HEPES, pH 7.4), in an incubator with 5% CO 2 and 95% atmospheric air at 37C

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Metformin activates AMPK, a key energy regulator that promotes autophagy, reduces senescent cells, and mimics the effects of caloric restrictionsupporting healthy aging and cellular repair