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doi: 10.3969/j.issn.1006-267x.2020.05.030 96

All collected samples were diluted to a protein concentration of 1 ng/l, and 5 l of each sample was loaded on a Tris-Glycine gel (Novex Tris-Glycine Mini Protein Gels, 1020%, 1.0 mm, WedgeWell) for Western blot analysis

The endogenous glucocorticoids bind to the mineralocorticoid receptors with a 10-fold greater affinity than to the glucocorticoid receptors (79-81)

Notably, the evaluation of discernment ability using receiver operating characteristic curve (ROC) analysis showed that kallistatin and transferrin exhibit greater discernment than conventional hormonal markers for identifying negative or positive conditions