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Beyond cell culture studies, the secretion of endogenous Humanin from human primary skeletal muscle cells was measured

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When Shh binds to the Ptc-1 receptor, inhibition of Smo is relieved, thereby activating Gli transcription factors and allowing Gli proteins to translocate from the cytoplasm into the nucleus, further mediating the downstream effects of the Shh signaling pathway [41]

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Fennema D, Phillips IR, Shephard EA