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In vitro embryo culture At 18 h post insemination (hpi), the putative zygotes were washed by repeated pipetting and transferred to 25 L drops (approximately one embryo per L) of culture medium (SOF supplemented with 3 mg/mL bovine serum albumin), covered with mineral oil and cultured until Day 8 post insemination (dpi) at 38.5 C in a humidified atmosphere with 5% CO 2 , 5% O 2 and 90% N 2 in air

We therefore postulated that IGF-1R mAbs could represent a viable therapeutic tool to target IGF-1 action, and potentially mimic the beneficial effects associated with diminished IGF-1 signaling observed in animal models

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GSH-dependent protein de-nitrosylation occurs through a nitrosylation exchange mechanism that generates GSNO, which can be a substrate of the enzyme S-nitrosoglutathione reductase (GSNOR) that finally produces GSSG and ammonia (NH 3 ) [38,39,40]

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