2025 Apr;242(4):717-724

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For serum preparation, blood samples were incubated for 1 h at room temperature and then centrifuged (1,950 x g, 15 min, Varifuge 3.0, Heraeus, Hanau, Germany) and stored at -80C prior to further analysis
HUVEC cells were stained with 0.5 M Calcein-AM and the fluorescence was detected by fluorescence microscope, and quantitative analysis of each image was performed using Image J 1.53t software
10.1046/j.1432-1033.2003.03947.x 5 BikleD