Consistent with these findings, Gene Set Enrichment Analysis (GSEA) revealed enrichment of the SenMayo 30 and ferroptosis pathways in aged oocytes, indicating their transcriptional activation during oocyte aging, which were significantly attenuated by uridine supplementation, thereby reducing senescence-associated transcriptional features (Fig
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Chromatographic separation of the target compounds was carried out using an ultra-high performance liquid chromatography (UHPLC) system (Thermo Fisher, Vanquish type, USA) equipped with ACQUITY UPLC BEH Amide (2.1 100 mm, 1.7 um
In summary, the mechanism of action of NAC involves (a) restoration of cellular antioxidant potential by increasing GSH levels, (b) elimination of free radicals, and (c) inhibition of proinflammatory factors and enzymes [30]