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As a result, they may require higher doses or more frequent administration to achieve the desired results

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The term "acylating" as used herein refers to the introduction of one or more acyl groups covalently bonded to the free amino groups of the protein or peptide

(2) performing gradient elution by taking a reverse-phase silica gel filler as a stationary phase, a salt solution with a pH value regulated to 7-9 by ammonia water as a mobile phase A and acetonitrile as a mobile phase B, collecting fractions, and performing rotary evaporation to remove a part of the solvent to obtain a primarily-purified solution