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Patch pipettes, prepared with a micropipette puller (Narishige, Tokyo, Japan), had resistances of 45 M for whole-cell recordings and 68 M for cell-attached recordings

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Although conducted in breast cancer cell lines, recent studies have confirmed similar metabolic processes in prostate cancer (77).These findings highlight tumor-specific adaptations in ammonium metabolism, emphasizing its role in sustaining proliferation and metabolic flexibility