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Briefly, Quercetin (0.09 g) was dissolved in 5 ml methanol, and this solution was added to cholesterol (0.06 g), sodium deoxycholate (0.08 g), and lecithin (0.4 g) that was dissolved in 10 ml chloroform in a round-bottom flask and then evaporated until dry in a rotary evaporator (Rota vapor, Heidolph VV 2000, Burladingen, Germany) at 100 rpm for 30 min at 25 C, forming a lipid thin film