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J Clin Diagn Res 8(11):CC01CC03

Screening of GST fragments which can inhibit the GST activity 150 single positive clones (grown on the RGB plates), that could tightly bind to the glutathione Sepharose 4B, were picked up from the plates, and cultured separately in 50 ml of IMC medium containing 100 g/ml ampicillin for 15 hours at 25C, then induced with 100 g/ml tryptophan for 6 hours

Free GHK has residual biological activity, but the majority of documented effects require the Cu(II) complex

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Cell death was shown by the change in cell viability, which was calculated as: (viable cell)% = (optical density (OD) of untreated sample/OD of treated sample) 100