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M.OrlandoP.SilvestriS.MxinwaV.MokgalaboniK.et al (2020)

-glucan training affects gene regulatory mechanisms, causing altered histone methylation (namely H3K4me1 enrichment), which leads to transcriptional induction of IL-32 by M to produce higher levels of antimicrobial peptides such as cathelicidin and -defensin 2 (168)

Protein function annotation based on heterogeneous biological networks

After centrifugation (1200 g, 10 min), the supernatant was discarded, and the resulting cell pellet was resuspended in culture medium supplemented with 5% of (FBS) (F4135, Sigma- Aldrich, Pozna, Poland) and seeded into cell culture flasks

Follow with an appropriate amount of serum, spreading gently according to skin texture 3