Samples were resuspended in 160 L EPPS buffer (200 mM, pH 8.0) using a probe sonicator (1 1015 pulses, power output 20%) and then digested with LysC (4 L of 0.5 g/L per sample, resuspended in HPLC grade water, Wako-chemicals, Fujifilm #125-05061) for 2 h at 37 C in a shaker incubator
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The use of oral or topical glutathione depends on the requirements and advice of healthcare professionals
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Le Gal K, Ibrahim MX, Wiel Cl, Sayin VI, Akula MK, Karlsson C, Dalin MG, Akyrek LM, Lindahl P, Nilsson J, Bergo MO