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Western Blot analysis Cell pellets were incubated with RIPA buffer (150mM sodium chloride, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulphate, 50mM Tris-HCl pH8) supplemented with protease inhibitors (Roche) for 45min then centrifuged at 17,000g for 10min and the supernatant was retained

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The culture medium was supplemented with 10% fetal bovine serum (Gibco, NY, USA) and 5% penicillin-streptomycin-gentamicin antibiotics (Solarbio, Beijing, China)