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This is a standard safety measure to prevent bacterial growth and ensure the medication remains sterile for injection

The column was washed with 20 mM HEPES, pH 7.5, 300 mM NaCl, 0.1% LMNG, 0.01% CHS, 5 mM imidazole, 1 mM TCEP and 20 mM HEPES, pH 7.5, 300 mM NaCl, 0.01% LMNG, 0.001% CHS, 20 mM imidazole, 1 mM TCEP

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Adiponectin safeguards cardiovascular health by exerting vasodilator, anti-apoptotic, anti-inflammatory, and anti-oxidative effects on both cardiac and vascular cells