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Unweighted UniFrac distance analysis, reflecting phylogenetic community structure, demonstrated the greatest phylogenetic dissimilarity in the VPA-ASD group relative to controls (Figure S4C)

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Regulation of glutaminase activity and glutamine metabolism

N-acetylcysteine, or NAC, is a supplement that works in two ways: It provides cysteine, which is the rate-limiting amino acid for glutathione production It can also act as a direct antioxidant to scavenge ROS (NO2 and HOX)[ref][ref] The main antioxidant mechanism for NAC is by increasing glutathione levels