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Negative-staining electron microscopy Purified GLP-2RG s complex was diluted to 0.012 mg/mL with 20 mM HEPES, pH 7.4, 100 mM NaCl, 5 M GLP-2, 100 M TCEP, 2 mM MgCl 2 , 0.00075% (w/v) LMNG, 0.00025% (w/v) GDN and 0.0002% (w/v) CHS

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For this reason, the glucosinolate pattern of 48 commercial cruciferous seeds was investigated, half of them (24 samples) with the declaration broccoli. Glucoraphanin could be detected in significant amounts in 15 of the samples

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