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Fibroblasts were cultured in T75 flasks in Dulbeccos Modified Eagles Medium (DMEM) supplemented with 10% foetal bovine serum (FBS), 50 units/ml penicillin, 50 g/ml streptomycin, 2 mM l-glutamine, 1 mM sodium pyruvate and 1 non-essential amino acids (Life Technologies, Paisley, UK), at 37 C and 5% CO 2

Oct-Dec 2019;31(4):555-560