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RNAi was performed by feeding adult staged worms (P0) bacterial expressing double stranded RNAs (dsRNAs) corresponding to the gene of interest as previously described (Kamath et al., 2001

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Tissue sections were observed under a Leica DMI-3000B microscope (Leica, Germany)

It's to minimize suppression depth and duration

The increased risk of bleeding with vitamin E appears to be dose-dependent