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5A, lane 4), suggesting that Ube2e2 that interact with DET1 may facilitate the initial modification of c-Jun with COP1 RING domain

Although these assays were conducted in aqueous solution and must be interpreted with caution, as the protein environment can further modulate the redox potential of flavins, this combined evidence supports a mechanism in which roseoflavin-derived metabolites incorporate into FSP1 but cannot accept electrons from NAD(P)H, thereby inactivating the enzyme