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& Stove, C

Buffers used for the various steps in the solubility of IBs LSB-1: 50 mM TrisCl pH 7.4, 1 mM EDTA, 10 mM -mercaptoethanol, 1% Triton-X 100, 300 mM NaCl, 1 mM Phenylmethanesulfonyl fluoride (PMSF) (Sigma, Catalogue-P7626) and 500 L BugBuster Protein Extraction Reagent (70,584 Merck-Millipore)

Furthermore, the validation parameters remained firmly within the acceptable range, as elegantly displayed in Table S1 (Supplementary materials)

Glutathione provides a fascinating fail-safe mechanism known as protein S-glutathionylation

Another thing to bear in mind is that some animal studies have cast doubt on NAC's ability to stimulate glutathione production and even called into question its overall safety