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Extracted proteins were denatured in 100 L of 6 M urea, reduced with 10 mM dithiothreitol (DTT) at room temperature for 60 min, and alkylated with 15 mM iodoacetamide in the dark for 60 min

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[DOI] [PMC free article] [PubMed] [Google Scholar] 43.Zhu S, Mason J, Shi Y, et al