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For subcellular localization of Nrf2, cytosolic and nuclear protein fractions were prepared using a commercial nuclear extraction kit (NE-PER , Thermo Fisher Scientific) according to the manufacturer's instructions

Mitigating the adverse effects of Lead and cadmium heavy metals-induced oxidative stress by phytogenic compounds in poultry

The Nrf2 activation along with the thiamine formation may explain why high doses of the thiamine disulfides, expected to decrease cellular redox potential, do not have any negative action even upon the long-term administration (155)