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The first example is citrate-derived acetyl-CoA, whose production is increased by PI3K/AKT-mediated ATP-citrate lyase (ACLY) enzyme [29]

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Samples were quenched in 1 mL pre-chilled 80C 80/20 methanol:water (v/v) and spiked with 50L 1M isotope labeled TCA cycle mix (Cambridge Isotope Laboratories MSK-TCA-A) pre-diluted in 80/20 methanol:water and 50L 0.02 ng/L Propionyl-L-carnitine-(N-methyl-d3) (Sigma Aldrich 52941)