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Furthermore, excessively high doses of standard ascorbic acid can increase urinary oxalate levels, raising the risk of kidney stones in susceptible individuals

Janet Sutcliffe, Winnipeg, Manitoba

The authors show that the drastic growth arrest of the sir1 mutant is partially rescued when glutathione deficiency is introduced via the cad2 mutation, which decreases glutathione levels to 30% to 40% of wild-type levels (Cobbett et al., 1998), or pharmacologically through treatment with the GCL inhibitor buthionine sulfoximine

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The final cell suspensions were analyzed by flow cytometry to test the intensity of DCFH-DA fluorescence at excitation and emission wavelengths of 480 nm and 530 nm, respectively