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Ge X, Zuo Y, Xie J, Li X, Li Y, Thirupathi A, Yu P, Gao G, Zhou C, Chang Y, Shi Z

Lysates were diluted in 2 Laemelli SDS sample buffer containing 50 mM DTT to equal concentration then ~ 10 g of protein electrophoresed through NuPage 38% TrisAcetate gels (Thermofisher Scientific) alongside Kaleidoscope (Bio-Rad, UK) and HiMark (Invitrogen, UK) protein standards

The erectile response induced by PT-141 was statistically significant at both doses

An imbalance between inflammatory factors and SPMs is prominent in facilitating formation of the vulnerable plaque