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|access-date= requires |url= (help) Cols Chacartegui R, Temprano Gonzlez R, Gmez Arruza C, Muoz Cacho P, Pascual Gmez J (2005)

In vitro transcription to prepare biotinylated PRKAA1 5UTR and scrambled RNA probes PRKAA1 5UTR and scrambled control RNA probes were synthesized using BamHI-linearized pUC57-T7 templates in an 80-l reaction buffer containing 5 transcription buffer (Roche, RPOLT7-RO), 8 l of 10 Dig labeling mixture (Merck, 11277073910), 8 l of 0.1 M DTT (Sigma, D9163

Coupled activity assay with Cp1B and Cp1D The coupled activity assay for Cp1B with Cp1D was typically performed in 100 l of 50 mM sodium phosphate buffer (pH 8.0) containing 25 M Cp1B, 25 M Cp1D, 5 mM ()- t -ES, 2.5 mM amino acid, 5 mM amine, 10 mM ATP cofactor and 10 mM MgCl 2

Q: Is it safe for long-term use