Because inhomogeneous cellular distribution of reacted and unreacted probes may bias the ratiometric calculations, especially at the subcellular resolution, we opted for RT instead of RT-NH 2 to achieve high-quality quantitative results in living cells
Per the existing literature, palmatine could attenuate LPS-induced inflammation by inhibiting ERK1/2, P38, and Akt/NF-kB signaling pathways (Ma et al., 2021)
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Frontiers in Pharmacology, 10 , 1172
Altogether, these findings indicate that the antioxidant effects of metformin are achieved by Nrf2 nuclear translocation, increased downstream cytoplasmic HO-1 expression, and the subsequent activation of Nrf2 dependent signaling pathway
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