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The quaternary structure practically exists thanks to presence of cysteine related disulfide bridges linking separate polypeptide chains

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Sprockett, D., Fukami, T

As a proxy for neuronal activity, neurons were exposed to anti-Syt1 lumenal primary antibody directly labeled with ATTO647N (1:200, 105 311AT1 clone 604.2 Synaptic Systems) in conditioned medium for 30 mins at 37 C