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The mobile phase consisted of HPLC grade acetonitrile and water with 0.1 % acetic acid

Studied extensively in wound healing and anti-aging skin models

Nature 428:569574 Mayer CM, Belsham DD (2010) Palmitate attenuates insulin signaling and induces endoplasmic reticulum stress and apoptosis in hypothalamic neurons: rescue of resistance and apoptosis through adenosine 5 monophosphateactivated protein kinase activation