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Then we discard the culture medium and add 100 l CCK-8 solution (diluted with ECM to 10% concentration) into each well, and incubated the 96-well plates for 2 h, and the absorbance of the cells at 450 nm was measured by the multifunctional reader FlexStation 3 (Molecular Devices, CA, USA)

These glycosides bind to sweet taste receptors on the tongue (specifically the T1R2/T1R3 receptor complex) with an intensity roughly 200-400 times greater than sucrose, which is why vanishingly small amounts produce noticeable sweetness

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Determination of B vitamins by double-vortex-ultrasonic assisted dispersive liquidliquid microextraction and evaluation of their possible roles in susceptibility to COVID 19 infection: Hybrid BoxBehnken design and genetic algorithm