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Aspiration before injection helps confirm the needle is not in a blood vessel

From the perspective of barrier function, A

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DNA mixture containing 26 g of transfer plasmid, 16.9 g of packaging plasmid (encoding viral Gag and Pol proteins (pCMVR8.91)), and 9.1 g of envelope plasmid (encoding the envelope of vesicular stomatitis virus (pCI)) and 78 l of 1 mg ml 1 polyethyleneimine in a total volume of 1.5 ml Dulbecco's modified eagle's medium was prepared