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Briefly, cells were lysed in the lysis buffer described above, and loaded onto a stepwise sucrose gradient composed of 700-l layers of sucrose of increasing percentage (top-to-bottom: 5%, 10%, 15%, 20%, 25%, and 30%)

As discussed by others, the role of autophagy in both tumor and immune cells can be paradoxical [34, 169], which adds another layer of complexity for the role of leucine in tumor biology