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Description

We found that therapeutic doses decrease liver GSH by only modest amounts (10%), but that overdoses of 10 grams or more severely deplete liver GSH (Figure6)

Pigment Cell

The GST construct (Amersham Biosciences) in pGEX-4T-3 vector was purified by manual glutathione-affinity chromatography using Glutathione Sepharose 4 fast flow beads (#17-5132-01, GE Healthcare) and eluted with 10 mM reduced glutathione (#11514, SRL), 150 mM NaCl and 2 mM DTT