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Purified stem cells from apical papilla (SCAPs) were seeded at 1 10 4 cells/mL into 10 cm culture dishes and cultured in alpha minimum essential medium (-MEM, Gibco, Life Technologies, Grand Island, NY) supplemented with 6% fetal bovine serum (Gibco, USA), 100 U/mL penicillin and 100 g/mL streptomycin at 37 C in 5% CO 2

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Zhang H, Wu F, Kong X, Yang J, Chen H, Deng L, Cheng Y, Ye L, Zhu S, Zhang X (2014) Nerve growth factor improves functional recovery by inhibiting endoplasmic reticulum stress-induced neuronal apoptosis in rats with spinal cord injury