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Western blot analysis Cells were washed with PBS containing 1 mmol L 1 N-Ethylmaleimide (NEM, Santa Cruz, #sc-202719), then lysed with Laemmli Sample buffer containing 2 % SDS, 4 % glycerol, 40 mmol L 1 Tris-Cl (pH 6.8), 5 % 2-Mercaptoethanol, 0.01 % bromophenol blue, 1 mmol L 1 NEM, 1 mmol L 1 Phenylmethylsulfonyl fluoride (PMSF), protease inhibitor mixture (Roche, #COEDTAF-RO), and phosphatase inhibitor mixture (Roche, #04906837001)

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GSH is a key antioxidant associated with the regeneration of ascorbic acid (AsA) in the AsAGSH cycle, which is an important pathway for clearance of reactive oxygen species (ROS) under kinds of stress ( 2 O 2 to H 2 O, thereby reducing or eliminating the toxicity of H 2 O 2

10.1002/nbm.3914 NMR Biomed

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