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known as GLT-1 in rodents) maintains glutamate homeostasis by clearing extracellular glutamate in exchange for Na + , K + , and H + ions
Counting was accomplished at 20X magnification and numerical aperture of 0.75, using a light/fluorescent microscope (Nikon Eclipse Ni, Nikon, USA) and Imaris software version 8.1.2 (Bitplane, Switzerland)
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