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The overall quality of evidence was evaluated using the GRADE (Grading of Recommendations, Assessment, Development and Evaluations) method

( I ) cAMP levels in cell lysates were determined following pretreatment with 100 nM each of GLP-1(2836), scrambled control, or forskolin in siRNA-treated cells (white bars) versus the scrambled siRNA control (gray bars) ( n = 3/group for all cAMP and LDH assays, each in triplicate)

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For example, whey protein helps in boosting the amino acid cysteine, which replenishes glutathione when its been used up by the immune process