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Interestingly, the affected patients did not have evidence of ongoing systemic carnitine deficiencies, although plasma and urine studies were able to clearly demonstrate abnormal carnitine metabolites

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Historically, these tests have served as key diagnostic tools in neurodegenerative diseases

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Then, samples were diluted with a solution containing phosphate buffer (0.01 mol/L monobasic potassium phosphate/dibasic potassium phosphate, pH 6.85) containing sodium dithionite, and after 10 min at room temperature, absorbance was measured at 420 and 432 nm against a matched curve containing only buffer [21]