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Over the past few years, medications such as glucagon-like peptide-1 have transformed the treatment of obesity and type 2 diabetes
Briefly, INS-1E cells were maintained in RPMI 1640 medium (Gibco) supplemented with 5% (v/v) heat-inactivated fetal bovine serum, 2 mM l-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 100 U/mL penicillin/streptomycin and 50 M 2-mercaptoethanol
Bake, uncovered, for 45 minutes or until a knife inserted into the center comes out clean
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