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For total ATP content measurements, cells were lysed with CellTiter-Glo Reagent (Promega Corporation, Madison, WI, USA) and total ATP determined

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(ii) Cu 2+ induces the secretion of acidic SMase from leukocytes which in turn leads to accumulation of erythrocytic ceramide [131] and (iii) treatment of erythrocytes with extracellular SMase resulted in ceramide-associated alterations in membrane-cytoskeleton interactions and membrane organization [125]

The hazard ratio (HR) for new-onset depression was 1.09 (95% CI, 1.041.14