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On the next day, targeting plasmids (sgRNA or overexpression) were co-transfected with pMD2.G (#12259, Addgene) and psPAX2 (#12260, Addgene) plasmids at 1:1:1 molar ratio using Lipofectamine 2000 (#11668019, Thermo Fisher) at 1:2.5 ratio

Patient education : Provide written and verbal instructions

You won't qualify if: Your GLP-1 medication is covered through Medicare Part D (MAPD, SNPs and PDP) and is used to treat: Type 2 diabetes: A health condition where your blood sugar is too high

Negative controls were processed for all assays and were made in the same manner except the incubation step with primary antibody, which was omitted

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