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The copper-binding capability is critical

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MTT Assay Normal and vitiligo melanocytes, treated with the autophagy inhibitor 3-methyl adenine as indicated, were then incubated with 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) (1 mg/mL) for 2 h at 37 C, and lysed in dimethyl sulfoxide (DMSO)